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pAAV-SMVP-Cas9N-U6-gRNA TdR
(Plasmid #80940)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 80940 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pZac2.1
  • Backbone manufacturer
    University of Pennsylvannia
  • Backbone size w/o insert (bp) 3000
  • Total vector size (bp) 7471
  • Vector type
    Mammalian Expression, AAV, CRISPR, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cas9N
  • Alt name
    Split-Cas9 N-terminal half with gRNA against Ai9 3xStop
  • Species
    Synthetic

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-SMVP-Cas9N-U6-gRNA TdR was a gift from George Church (Addgene plasmid # 80940 ; http://n2t.net/addgene:80940 ; RRID:Addgene_80940)

  • For your References section:

    A multifunctional AAV-CRISPR-Cas9 and its host response. Chew WL, Tabebordbar M, Cheng JK, Mali P, Wu EY, Ng AH, Zhu K, Wagers AJ, Church GM. Nat Methods. 2016 Sep 5. doi: 10.1038/nmeth.3993. 10.1038/nmeth.3993 PubMed 27595405
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