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Addgene

pTL032
(Plasmid #80928)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 80928 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR4
  • Backbone size w/o insert (bp) 2922
  • Total vector size (bp) 6152
  • Vector type
    Bacterial Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    14xMS2 sense + 14xPP7 antisense, loxP-kanMX-loxP
  • Insert Size (bp)
    1642

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The 14xMS2 stem loop cassette is in the sense direction, the 14xPP7 cassette is in the antisense direction. The plasmid also includes a loxP-kanMX-loxP sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTL032 was a gift from Daniel Larson (Addgene plasmid # 80928 ; http://n2t.net/addgene:80928 ; RRID:Addgene_80928)

  • For your References section:

    Single-Molecule Imaging Reveals a Switch between Spurious and Functional ncRNA Transcription. Lenstra TL, Coulon A, Chow CC, Larson DR. Mol Cell. 2015 Nov 19;60(4):597-610. doi: 10.1016/j.molcel.2015.09.028. Epub 2015 Nov 5. 10.1016/j.molcel.2015.09.028 PubMed 26549684
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