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pJPC12-ΔM13-mCherry-P/PM,5G6T-GFP
(Plasmid #80912)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 80912 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJPC12
  • Total vector size (bp) 6227
  • Modifications to backbone
    Deletion of M13 packaging signal
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha ; TOP 10
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    mCherry
  • Species
    Synthetic
  • Insert Size (bp)
    711
  • Promoter P5G6T

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    GFP
  • Species
    Synthetic
  • Insert Size (bp)
    717
  • Promoter PM,5G6T

Cloning Information for Gene/Insert 2

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The pJPC12 vector was obtained from Peterson and Phillips (Peterson, J. and G.J. Phillips, New pSC101-derivative cloning vectors with elevated copy numbers. Plasmid, 2008. 59(3): p. 193-201)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJPC12-ΔM13-mCherry-P/PM,5G6T-GFP was a gift from Mark Isalan (Addgene plasmid # 80912 ; http://n2t.net/addgene:80912 ; RRID:Addgene_80912)

  • For your References section:

    Engineering orthogonal dual transcription factors for multi-input synthetic promoters. Brodel AK, Jaramillo A, Isalan M. Nat Commun. 2016 Dec 16;7:13858. doi: 10.1038/ncomms13858. 10.1038/ncomms13858 PubMed 27982027
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