pJPC12-ΔM13-mCherry-P/PM,5G6G-GFP
(Plasmid #80911)

• Purpose: Reporter plasmid (mCherry and GFP)
• Depositing Lab: Mark Isalan
• Publication: Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10.1038/ncomms13858.

Backbone

• Vector backbone: pJPC12
• Total vector size (bp): 6227
• Modifications to backbone: Deletion of M13 packaging signal
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha ; TOP 10
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: mCherry
• Species: Synthetic
• Insert Size (bp): 711
• Promoter: P5G6G

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: AGCCGTACATGAACTGAG

Gene/Insert 2

• Gene/Insert name: GFP
• Species: Synthetic
• Insert Size (bp): 717
• Promoter: PM,5G6G

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: AGCCGTACATGAACTGAG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The pJPC12 vector was obtained from Peterson and Phillips (Peterson, J. and G.J. Phillips, New pSC101-derivative cloning vectors with elevated copy numbers. Plasmid, 2008. 59(3): p. 193-201)