pLITMUS-rpoN-cI4A5C6G7G-J23106-geneIII
(Plasmid #80909)

• Purpose: Phagemid (encodes cI4A5C6G7G and M13 gene III)
• Depositing Lab: Mark Isalan
• Publication: Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10.1038/ncomms13858.

Backbone

• Vector backbone: pLITMUS
• Backbone manufacturer: NEB
• Total vector size (bp): 4924
• Modifications to backbone: M13 gene III expression cassette cI4A5C6G7G expression cassette
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: M13 gene III
• Species: M13 bacteriophage
• Insert Size (bp): 1275
• Mutation: 238G to 238D (please see depositor comment below)
• Promoter: BBa_J23106

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CGTAGTTATCTACACGACG
• 3′ sequencing primer: TGCTTATACAATCTTCCTG

Gene/Insert 2

• Gene/Insert name: cI4A5C6G7G
• Species: Synthetic
• Insert Size (bp): 714
• Promoter: rpoN

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BsaI (not destroyed)
• 5′ sequencing primer: gtc gat ttt tgt gat gct cg
• 3′ sequencing primer: ggg tta ttg tct cat gag cgg ata c

Resource Information

• Supplemental Documents:
  • pLITMUS-rpoN-cI4A5C6G7G-J23106-geneIII.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that mutation 238G to 238D in M13 gene III was found during Addgene's quality control. The depositor noted that this mutation does NOT affect plasmid function.