pLITMUS-rpoN-cI4A5T6T-J23106-geneIII
(Plasmid #80908)

• Purpose: Phagemid (encodes cI4A5T6T and M13 gene III)
• Depositing Lab: Mark Isalan
• Publication: Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10.1038/ncomms13858.

Backbone

• Vector backbone: pLITMUS
• Backbone manufacturer: NEB
• Total vector size (bp): 4924
• Modifications to backbone: M13 gene III expression cassette cI4A5T6T expression cassette
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: M13 gene III
• Species: M13 bacteriophage
• Insert Size (bp): 1275
• Promoter: BBa_J23106

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CGTAGTTATCTACACGACG
• 3′ sequencing primer: TGCTTATACAATCTTCCTG

Gene/Insert 2

• Gene/Insert name: cI4A5T6T
• Species: Synthetic
• Insert Size (bp): 714
• Promoter: rpoN

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BsaI (not destroyed)
• 5′ sequencing primer: gtc gat ttt tgt gat gct cg
• 3′ sequencing primer: ggg tta ttg tct cat gag cgg ata c

Resource Information

• Supplemental Documents:
  • pLITMUS-rpoN-cI4A5T6T-J23106-geneIII

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene QC NGS identifies a SNP at nucleotide 1471 in the M13 ori. It is unknown what, if any, impact this may have on plasmid function.