FCK-Chronos-GFP
(Plasmid #80902)

• Purpose: Lenti-mediated expression of Chronos-GFP under the CaMKII promoter.
• Depositing Lab: Edward Boyden
• Publication: Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi: 10.1038/nmeth.2836. Epub 2014 Feb 9.

Backbone

• Vector backbone: FCK (1.3)GW
• Backbone manufacturer: Pavel Osten
• Backbone size w/o insert (bp): 9240
• Total vector size (bp): 10947
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Use recombinase-free E. coli (e.g., Invitrogen's Stbl3).
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Chronos-GFP
• Alt name: Stigeoclonium helveticum channelrhodopsin-GFP
• Alt name: ChR90-GFP
• Species: Stigeoclonium helveticum
• Insert Size (bp): 1707
• GenBank ID: KF992040
• Promoter: CaMKII
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: GCTGTGAGCAGCCACAGTG
• 3′ sequencing primer: GCAATAGCATGATACAAAGGC

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

PLEASE CONTACT ED BOYDEN () FOR DETAILS ON THIS REAGENT AND FURTHER REAGENTS IN THIS LINE.