pcDNA3-Flag-Smad4 R100T
(Plasmid #80891)

• Purpose: mammalian expression of Smad4 R100T
• Depositing Lab: Aristidis Moustakas
• Publication: Moren et al Oncogene. 2000 Sep 7;19(38):4396-404.

Backbone

• Vector backbone: pcDNA3
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Smad4
• Alt name: SMAD family member 4
• Species: H. sapiens (human)
• Mutation: Pancreatic cancer mutation R100T
• Entrez Gene: SMAD4 (a.k.a. DPC4, JIP, MADH4, MYHRS)
• Promoter: CMV
• Tag / Fusion Protein:
  • Flag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid contains a D2E mutation in SMAD4 compared to NM_005359.5. The depositor states that this mutation does not affect plasmid function.