pcDNA3-ALK5 T204D
(Plasmid #80877)

• Purpose: mammalian expression of constitutively active ALK5 T204D
• Depositing Lab: Aristidis Moustakas
• Publication: Moren et al J Biol Chem. 2005 Jun 10;280(23):22115-23. Epub 2005 Apr 6.

Backbone

• Vector backbone: pcDNA3
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ALK5
• Alt name: TGFBR1
• Alt name: transforming growth factor beta receptor 1
• Species: H. sapiens (human)
• Mutation: T204D (constitutively active)
• Entrez Gene: TGFBR1 (a.k.a. AAT5, ACVRLK4, ALK-5, ALK5, ESS1, LDS1, LDS1A, LDS2A, MSSE, SKR4, TBR-i, TBRI, TGFR-1, tbetaR-I)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry