pcDNA3-ALK3 K261R
(Plasmid #80875)

• Purpose: mammalian expression of ALK3 K261R
• Depositing Lab: Aristidis Moustakas
• Publication: Raja et al Oncotarget. 2016 Jan 12;7(2):1120-43. doi: 10.18632/oncotarget.6683.

Backbone

• Vector backbone: pcDNA3
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ALK3
• Alt name: BMPR1A
• Alt name: bone morphogenetic protein receptor type 1A
• Species: H. sapiens (human)
• Mutation: K261R (Kinase inactive)
• Entrez Gene: BMPR1A (a.k.a. 10q23del, ACVRLK3, ALK-3, ALK3, BMPR-1A, CD292, SKR5)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid contains a P2A mutation compared to NM_004329.2 and SL insertion after the c-terminal HA tag. The depositor states that these discrepancies do not affect plasmid function.