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PurposeAccessory plasmid (encodes M13 gene VI)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 80858 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepJPC12
- Total vector size (bp) 5300
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Modifications to backboneDeletion of M13 packaging signal
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha ; TOP 10
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameM13 gene VI
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SpeciesM13 bacteriophage
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Insert Size (bp)339
- Promoter Lambda Prm
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer AAA CGA CGG CCA GTG AGC
- 3′ sequencing primer G ATA ACA ATT TCA CAC AGG (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The pJPC12 vector was obtained from Peterson and Phillips (Peterson, J. and G.J. Phillips, New pSC101-derivative cloning vectors with elevated copy numbers. Plasmid, 2008. 59(3): p. 193-201)
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJPC12-ΔM13-PRM-B0034-geneVI was a gift from Mark Isalan (Addgene plasmid # 80858 ; http://n2t.net/addgene:80858 ; RRID:Addgene_80858) -
For your References section:
Engineering orthogonal dual transcription factors for multi-input synthetic promoters. Brodel AK, Jaramillo A, Isalan M. Nat Commun. 2016 Dec 16;7:13858. doi: 10.1038/ncomms13858. 10.1038/ncomms13858 PubMed 27982027
