pScarlessHD-2xHA-DsRed
(Plasmid #80822)

• Purpose: Vector containing a cassette for scarless insertion of 2xHA and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.
• Depositing Lab: Kate O'Connor-Giles
• Publication: Kate O'Connor-Giles Lab Donor Plasmids (unpublished)

Backbone

• Vector backbone: pCR™4-TOPO
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 3956
• Total vector size (bp): 5793
• Vector type: Unspecified

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Kanamycin, 100 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 2xHA-piggyBac-DsRed
• Alt name: Linker-2xHA-pBac-DsRed-Linker
• Species: Synthetic
• Insert Size (bp): 1837
• Tag / Fusion Protein:
  • 2xHA

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T3

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Some sequences were synthesized by DNA 2.0
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry