p3E-mKate2-myc no-pA
(Plasmid #80812)

• Purpose: myc epitope fused to C-terminus of mKate2 without pA
• Depositing Lab: Philip Washbourne
• Publication: Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi: 10.1371/journal.pone.0159277. eCollection 2016.

Backbone

• Vector backbone: pDONR P2R-P3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 2619
• Total vector size (bp): 3370
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mKateHA p3E
• Insert Size (bp): 751

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13(20)F
• 3′ sequencing primer: M13R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Evergen

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry