pDHK29
(Plasmid
#80783)
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Purpose(Empty Backbone) Cloning vector compatible with ColE1 plasmids with high copy number
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 80783 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepWSK29
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Backbone manufacturerWang, R.F. and S. R. Kushner. 1991. Gene 100:195-199.
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Cloning Information
- Cloning method Restriction Enzyme
- 3′ sequencing primer M13 reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDHK29 was a gift from Gregory Phillips (Addgene plasmid # 80783 ; http://n2t.net/addgene:80783 ; RRID:Addgene_80783) -
For your References section:
High copy number plasmids compatible with commonly used cloning vectors. Phillips GJ, Park SK, Huber D. Biotechniques. 2000 Mar;28(3):400-2, 404, 406 passim. 10.2144/00283bm02 PubMed 10723548
Map uploaded by the depositor.
