peSpCas9(1.1)-2×sgRNA (empty, donor) Citations (17)
Originally described in: Practical method for targeted disruption of cilia-related genes by using CRISPR/Cas9-mediated homology-independent knock-in system.Katoh Y, Michisaka S, Nozaki S, Funabashi T, Hirano T, Takei R, Nakayama K Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-01-0051. doi: 10.1091/mbc.E17-01-0051. PubMed Journal
Articles Citing peSpCas9(1.1)-2×sgRNA (empty, donor)
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| Cystine uptake through the cystine/glutamate antiporter xCT triggers glioblastoma cell death under glucose deprivation. Goji T, Takahara K, Negishi M, Katoh H. J Biol Chem. 2017 Dec 1;292(48):19721-19732. doi: 10.1074/jbc.M117.814392. Epub 2017 Oct 16. PubMed |
| Ciliopathy-associated mutations of IFT122 impair ciliary protein trafficking but not ciliogenesis. Takahara M, Katoh Y, Nakamura K, Hirano T, Sugawa M, Tsurumi Y, Nakayama K. Hum Mol Genet. 2018 Feb 1;27(3):516-528. doi: 10.1093/hmg/ddx421. PubMed |
| BBS1 is involved in retrograde trafficking of ciliary GPCRs in the context of the BBSome complex. Nozaki S, Katoh Y, Kobayashi T, Nakayama K. PLoS One. 2018 Mar 28;13(3):e0195005. doi: 10.1371/journal.pone.0195005. eCollection 2018. PubMed |
Robust interaction of IFT70 with IFT52-IFT88 in the IFT-B complex is required for ciliogenesis.
Takei R, Katoh Y, Nakayama K.
Biol Open. 2018 Apr 30;7(5). pii: bio.033241. doi: 10.1242/bio.033241.
PubMed
Associated Plasmids |
| Interaction of WDR60 intermediate chain with TCTEX1D2 light chain of the dynein-2 complex is crucial for ciliary protein trafficking. Hamada Y, Tsurumi Y, Nozaki S, Katoh Y, Nakayama K. Mol Biol Cell. 2018 Jul 1;29(13):1628-1639. doi: 10.1091/mbc.E18-03-0173. Epub 2018 May 9. PubMed |
| EphA3 is up-regulated by epidermal growth factor and promotes formation of glioblastoma cell aggregates. Toyama M, Hamaoka Y, Katoh H. Biochem Biophys Res Commun. 2019 Jan 15;508(3):715-721. doi: 10.1016/j.bbrc.2018.12.002. Epub 2018 Dec 6. PubMed |
| Interactions of the dynein-2 intermediate chain WDR34 with the light chains are required for ciliary retrograde protein trafficking. Tsurumi Y, Hamada Y, Katoh Y, Nakayama K. Mol Biol Cell. 2019 Mar 1;30(5):658-670. doi: 10.1091/mbc.E18-10-0678. Epub 2019 Jan 16. PubMed |
| The cystine/glutamate antiporter xCT is a key regulator of EphA2 S897 phosphorylation under glucose-limited conditions. Teramoto K, Katoh H. Cell Signal. 2019 Oct;62:109329. doi: 10.1016/j.cellsig.2019.05.014. Epub 2019 May 29. PubMed |
| Requirement of IFT-B-BBSome complex interaction in export of GPR161 from cilia. Nozaki S, Castro Araya RF, Katoh Y, Nakayama K. Biol Open. 2019 Sep 17;8(9). pii: bio.043786. doi: 10.1242/bio.043786. PubMed |
| Formation of the B9-domain protein complex MKS1-B9D2-B9D1 is essential as a diffusion barrier for ciliary membrane proteins. Okazaki M, Kobayashi T, Chiba S, Takei R, Liang L, Nakayama K, Katoh Y. Mol Biol Cell. 2020 Sep 15;31(20):2259-2268. doi: 10.1091/mbc.E20-03-0208. Epub 2020 Jul 29. PubMed |
| Interaction of INPP5E with ARL13B is essential for its ciliary membrane retention but dispensable for its ciliary entry. Qiu H, Fujisawa S, Nozaki S, Katoh Y, Nakayama K. Biol Open. 2021 Jan 25;10(1). pii: bio.057653. doi: 10.1242/bio.057653. PubMed |
| Role of ferritinophagy in cystine deprivation-induced cell death in glioblastoma cells. Hayashima K, Kimura I, Katoh H. Biochem Biophys Res Commun. 2021 Feb 5;539:56-63. doi: 10.1016/j.bbrc.2020.12.075. Epub 2021 Jan 6. PubMed |
| Combinations of deletion and missense variations of the dynein-2 DYNC2LI1 subunit found in skeletal ciliopathies cause ciliary defects. Qiu H, Tsurumi Y, Katoh Y, Nakayama K. Sci Rep. 2022 Jan 7;12(1):31. doi: 10.1038/s41598-021-03950-0. PubMed |
| Expression of gamma-glutamyltransferase 1 in glioblastoma cells confers resistance to cystine deprivation-induced ferroptosis. Hayashima K, Katoh H. J Biol Chem. 2022 Mar;298(3):101703. doi: 10.1016/j.jbc.2022.101703. Epub 2022 Feb 8. PubMed |
| Dynein-2-driven intraciliary retrograde trafficking indirectly requires multiple interactions of IFT54 in the IFT-B complex with the dynein-2 complex. Hiyamizu S, Qiu H, Tsurumi Y, Hamada Y, Katoh Y, Nakayama K. Biol Open. 2023 Jun 13;12(7):bio059976. doi: 10.1242/bio.059976. PubMed |
| CD44 correlates with longevity and enhances basal ATF6 activity and ER stress resistance. Takasugi M, Ohtani N, Takemura K, Emmrich S, Zakusilo FT, Yoshida Y, Kutsukake N, Mariani JN, Windrem MS, Chandler-Militello D, Goldman SA, Satoh J, Ito S, Seluanov A, Gorbunova V. Cell Rep. 2023 Sep 26;42(9):113130. doi: 10.1016/j.celrep.2023.113130. Epub 2023 Sep 13. PubMed |
| Variants in the WDR44 WD40-repeat domain cause a spectrum of ciliopathy by impairing ciliogenesis initiation. Accogli A, Shakya S, Yang T, Insinna C, Kim SY, Bell D, Butov KR, Severino M, Niceta M, Scala M, Lee HS, Yoo T, Stauffer J, Zhao H, Fiorillo C, Pedemonte M, Diana MC, Baldassari S, Zakharova V, Shcherbina A, Rodina Y, Fagerberg C, Roos LS, Wierzba J, Dobosz A, Gerard A, Potocki L, Rosenfeld JA, Lalani SR, Scott TM, Scott D, Azamian MS, Louie R, Moore HW, Champaigne NL, Hollingsworth G, Torella A, Nigro V, Ploski R, Salpietro V, Zara F, Pizzi S, Chillemi G, Ognibene M, Cooney E, Do J, Linnemann A, Larsen MJ, Specht S, Walters KJ, Choi HJ, Choi M, Tartaglia M, Youkharibache P, Chae JH, Capra V, Park SG, Westlake CJ. Nat Commun. 2024 Jan 8;15(1):365. doi: 10.1038/s41467-023-44611-2. PubMed |
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