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Addgene

pDonor-tBFP-NLS-Neo
(Plasmid #80766)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 80766 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTagBFP-C
  • Backbone manufacturer
    Evrogen
  • Backbone size (bp) 4700
  • Modifications to backbone
    A triplicated nuclear localization signal (NLS) was fused to tBFP. Cleavage sites for the restriction enzyme BbsI/BpiI were added on upstream of the cytomegalovirus promoter to enable insertion of the target DNA sequence.
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Neomycin (select with G418)
  • Tag / Fusion Protein
    • TagBFP2-3×NLS

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDonor-tBFP-NLS-Neo was a gift from Kazuhisa Nakayama (Addgene plasmid # 80766 ; http://n2t.net/addgene:80766 ; RRID:Addgene_80766)

  • For your References section:

    Practical method for targeted disruption of cilia-related genes by using CRISPR/Cas9-mediated homology-independent knock-in system. Katoh Y, Michisaka S, Nozaki S, Funabashi T, Hirano T, Takei R, Nakayama K. Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-01-0051. doi: 10.1091/mbc.E17-01-0051. 10.1091/mbc.E17-01-0051 PubMed 28179459
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