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Addgene

pBABE-hygro-mTurquoise2-Nuf2
(Plasmid #80762)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 80762 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBABE-hygro
  • Backbone size w/o insert (bp) 5542
  • Total vector size (bp) 7693
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nuf2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1413
  • GenBank ID
    NM_145697.2
  • Entrez Gene
    NUF2 (a.k.a. CDCA1, CT106, NUF2R)
  • Tag / Fusion Protein
    • mTurquoise2 (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CAT GGT CCT GCT GGA GTT CGT G
  • 3′ sequencing primer ACCCTAACTGACACACATTCC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABE-hygro-mTurquoise2-Nuf2 was a gift from Daniel Needleman (Addgene plasmid # 80762 ; http://n2t.net/addgene:80762 ; RRID:Addgene_80762)

  • For your References section:

    Measuring NDC80 binding reveals the molecular basis of tension-dependent kinetochore-microtubule attachments. Yoo TY, Choi JM, Conway W, Yu CH, Pappu RV, Needleman DJ. Elife. 2018 Jul 25;7. pii: 36392. doi: 10.7554/eLife.36392. 36392 [pii] PubMed 30044223
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