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pBS-EF1-IRES-NEO-PSS1
(Plasmid #80759)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 80759 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBS-KS plus (BlueScribe KS Plus)
  • Backbone size w/o insert (bp) 2964
  • Total vector size (bp) 6345
  • Modifications to backbone
    Replaced PB-EF1-IRES NEO Inverted Terminal Repeats with PSS1 Inverted Terminal Repeats around NeoR (CTGCATTCCAG / CTGGAATGCAG)
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NeoR
  • Species
    Synthetic
  • Entrez Gene
    neoR (a.k.a. pSH111_227_223)
  • Promoter EF1-IRES from PB-EF1-IRES-NEO

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS-EF1-IRES-NEO-PSS1 was a gift from Alex Kentsis (Addgene plasmid # 80759)

  • For your References section:

    PGBD5 promotes site-specific oncogenic mutations in human tumors. Henssen AG, Koche R, Zhuang J, Jiang E, Reed C, Eisenberg A, Still E, MacArthur IC, Rodriguez-Fos E, Gonzalez S, Puiggros M, Blackford AN, Mason CE, de Stanchina E, Gonen M, Emde AK, Shah M, Arora K, Reeves C, Socci ND, Perlman E, Antonescu CR, Roberts CWM, Steen H, Mullen E, Jackson SP, Torrents D, Weng Z, Armstrong SA, Kentsis A. Nat Genet. 2017 May 15. doi: 10.1038/ng.3866. 10.1038/ng.3866 PubMed 28504702
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