mbYFPQS
(Plasmid #80742)

• Purpose: membrane-targeted biosensor for monitoring changes in intracellular chloride
• Depositing Labs: Susan Amara, Susan Ingram
• Publication: Watts et al PLoS One. 2012;7(4):e35373. doi: 10.1371/journal.pone.0035373. Epub 2012 Apr 10.

Backbone

• Vector backbone: pEYFP-Mem
• Backbone manufacturer: Clontech
• Total vector size (bp): 4800
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: YFPQS
• Alt name: YFP with enhanced sensitivity to low chloride concentrations (YFPQS)
• Species: Synthetic
• Mutation: H148Q (CAC to CAG) and V163S (GTG to AGC).
• Promoter: CMV
• Tag / Fusion Protein:
  • palmitoylation signal sequence of neuromodulin (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry