Skip to main content
Addgene

mbYFPQS
(Plasmid #80742)

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 80742 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pEYFP-Mem
  • Backbone manufacturer
    Clontech
  • Total vector size (bp) 4800
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    YFPQS
  • Alt name
    YFP with enhanced sensitivity to low chloride concentrations (YFPQS)
  • Species
    Synthetic
  • Mutation
    H148Q (CAC to CAG) and V163S (GTG to AGC).
  • Promoter CMV
  • Tag / Fusion Protein
    • palmitoylation signal sequence of neuromodulin (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mbYFPQS was a gift from Susan Amara & Susan Ingram (Addgene plasmid # 80742 ; http://n2t.net/addgene:80742 ; RRID:Addgene_80742)
  • For your References section:

    A sensitive membrane-targeted biosensor for monitoring changes in intracellular chloride in neuronal processes. Watts SD, Suchland KL, Amara SG, Ingram SL. PLoS One. 2012;7(4):e35373. doi: 10.1371/journal.pone.0035373. Epub 2012 Apr 10. 10.1371/journal.pone.0035373 PubMed 22506078