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Addgene

pCDB294
(Plasmid #80693)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 80693 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDB180
  • Backbone manufacturer
    custom made
  • Backbone size w/o insert (bp) 6155
  • Total vector size (bp) 6284
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    gHEEE_02
  • Alt name
    HEEE_2.2_02
  • Species
    Synthetic
  • Insert Size (bp)
    129
  • Promoter T7
  • Tag / Fusion Protein
    • OsmY-10His-Smt3 (N terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CCTGTATGACGGTATTCG
  • 3′ sequencing primer GCTAGTTATTGCTCAGCGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDB294 was a gift from David Baker (Addgene plasmid # 80693 ; http://n2t.net/addgene:80693 ; RRID:Addgene_80693)

  • For your References section:

    Accurate de novo design of hyperstable constrained peptides. Bhardwaj G, Mulligan VK, Bahl CD, Gilmore JM, Harvey PJ, Cheneval O, Buchko GW, Pulavarti SV, Kaas Q, Eletsky A, Huang PS, Johnsen WA, Greisen PJ, Rocklin GJ, Song Y, Linsky TW, Watkins A, Rettie SA, Xu X, Carter LP, Bonneau R, Olson JM, Coutsias E, Correnti CE, Szyperski T, Craik DJ, Baker D. Nature. 2016 Sep 14. doi: 10.1038/nature19791. 10.1038/nature19791 PubMed 27626386
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