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pEN-L1-K2-L2
(Plasmid #80684)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 80684 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR201
  • Backbone manufacturer
    invitrogen
  • Vector type
    Mammalian Expression, Bacterial Expression, Yeast Expression, Insect Expression, Plant Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    DHFR
  • Species
    M. musculus (mouse)
  • Mutation
    mouse DHFR with N-terminal Ubiquitin
  • GenBank ID
    NM_010049 NM_010049
  • Entrez Gene
    Dhfr (a.k.a. 8430436I03Rik)
  • Tag / Fusion Protein
    • 3xHA (C terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Gateway Cloning
  • 5′ sequencing primer ctcgagctgcagaattactatttacaattaca
  • 3′ sequencing primer AGCACCAGCACCAGCGTAATCTGGAACGTCGTATGGATA
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    DHFR
  • Species
    M. musculus (mouse), A. thaliana (mustard weed)
  • Mutation
    mouse DHFR with N-terminal Ubiquitin
  • GenBank ID
    NM_010049.3
  • Entrez Gene
    Dhfr (a.k.a. 8430436I03Rik)
  • Tag / Fusion Protein
    • 3xHA (C terminal on insert)

Cloning Information for Gene/Insert 2

  • Cloning method Gateway Cloning
  • 5′ sequencing primer ctcgagctgcagaattactatttacaattaca
  • 3′ sequencing primer AGCACCAGCACCAGCGTAATCTGGAACGTCGTATGGATA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEN-L1-K2-L2 was a gift from Nico Dissmeyer (Addgene plasmid # 80684 ; http://n2t.net/addgene:80684 ; RRID:Addgene_80684)

  • For your References section:

    Phenotypes on demand via switchable target protein degradation in multicellular organisms. Faden F, Ramezani T, Mielke S, Almudi I, Nairz K, Froehlich MS, Hockendorff J, Brandt W, Hoehenwarter W, Dohmen RJ, Schnittger A, Dissmeyer N. Nat Commun. 2016 Jul 22;7:12202. doi: 10.1038/ncomms12202. 10.1038/ncomms12202 PubMed 27447739
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