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Purpose(Empty Backbone) for secretion of N-terminal OsmY, deca-histidine, SUMO tagged protein
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 80677 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCDB180
- Backbone size (bp) 6155
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Vector typeBacterial Expression
- Promoter T7
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Tag
/ Fusion Protein
- OsmY-10His-Smt3 (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CCTGTATGACGGTATTCG
- 3′ sequencing primer GCTAGTTATTGCTCAGCGG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This is an empty expression vector. Genes are intended to be inserted at the XhoI site by Gibson assembly, and this will generate constructs that are secreted with a N-terminal fusion to OsmY-10His-Smt3
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDB180 was a gift from David Baker (Addgene plasmid # 80677 ; http://n2t.net/addgene:80677 ; RRID:Addgene_80677) -
For your References section:
Accurate de novo design of hyperstable constrained peptides. Bhardwaj G, Mulligan VK, Bahl CD, Gilmore JM, Harvey PJ, Cheneval O, Buchko GW, Pulavarti SV, Kaas Q, Eletsky A, Huang PS, Johnsen WA, Greisen PJ, Rocklin GJ, Song Y, Linsky TW, Watkins A, Rettie SA, Xu X, Carter LP, Bonneau R, Olson JM, Coutsias E, Correnti CE, Szyperski T, Craik DJ, Baker D. Nature. 2016 Sep 14. doi: 10.1038/nature19791. 10.1038/nature19791 PubMed 27626386
