pENTR-SYNZIP12
(Plasmid
#80667)
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PurposeEncodes the synthetic coiled coil peptide SYNZIP12
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 80667 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepENTR_D-TOPO
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2586
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Vector typeEntry vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSYNZIP12
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SpeciesSynthetic
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Insert Size (bp)129
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer M13 (-21) Forward, TGTAAAACGACGGCCAGT
- 3′ sequencing primer M13 Reverse, CAGGAAACAGCTATGAC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENTR-SYNZIP12 was a gift from Amy Keating (Addgene plasmid # 80667 ; http://n2t.net/addgene:80667 ; RRID:Addgene_80667) -
For your References section:
SYNZIP protein interaction toolbox: in vitro and in vivo specifications of heterospecific coiled-coil interaction domains. Thompson KE, Bashor CJ, Lim WA, Keating AE. ACS Synth Biol. 2012 Apr 20;1(4):118-29. 10.1021/sb200015u PubMed 22558529