pQLinkHD-SYNZIP4
(Plasmid
#80660)
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PurposeEncodes the synthetic coiled coil peptide SYNZIP4
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 80660 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepQE-2
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Backbone manufacturerQiagen
- Backbone size w/o insert (bp) 4892
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSYNZIP4
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SpeciesSynthetic
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Insert Size (bp)165
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Tag
/ Fusion Protein
- 6xHis (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer pQE65, TGAGCGGATAACAATTTCACACAG
- 3′ sequencing primer pQE276, GGCAACCGAGCGTTCTGAAC (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pQLinkHD-SYNZIP4 was a gift from Amy Keating (Addgene plasmid # 80660 ; http://n2t.net/addgene:80660 ; RRID:Addgene_80660) -
For your References section:
SYNZIP protein interaction toolbox: in vitro and in vivo specifications of heterospecific coiled-coil interaction domains. Thompson KE, Bashor CJ, Lim WA, Keating AE. ACS Synth Biol. 2012 Apr 20;1(4):118-29. 10.1021/sb200015u PubMed 22558529
Map uploaded by the depositor.
