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Addgene

pProm1_BCD20-GFP
(Plasmid #80651)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 80651 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    N/A
  • Backbone manufacturer
    N/A
  • Total vector size (bp) 2353
  • Modifications to backbone
    n/a
  • Vector type
    p15A origin, constitutive promoter, KanR
  • Selectable markers
    Kanamycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Growth instructions
    Medium copy
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GFP
  • Alt name
    sfGFP
  • Insert Size (bp)
    717
  • Mutation
    NONE
  • Promoter constitutive promoter

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer QB3284_Fwd CGATCCTCATCCTGTCTCTTGATC
  • 3′ sequencing primer QB3810_Rev CGAGCGTAGCGAGTCAGTG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pProm1_BCD20-GFP was a gift from Nathan Hillson (Addgene plasmid # 80651 ; http://n2t.net/addgene:80651 ; RRID:Addgene_80651)

  • For your References section:

    PR-PR: cross-platform laboratory automation system. Linshiz G, Stawski N, Goyal G, Bi C, Poust S, Sharma M, Mutalik V, Keasling JD, Hillson NJ. ACS Synth Biol. 2014 Aug 15;3(8):515-24. doi: 10.1021/sb4001728. Epub 2014 Jan 14. 10.1021/sb4001728 PubMed 25126893
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