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Addgene

pQLinkHD-SYNZIP1
(Plasmid #80647)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 80647 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pQE-2
  • Backbone manufacturer
    Qiagen
  • Backbone size w/o insert (bp) 4892
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SYNZIP1
  • Species
    Synthetic
  • Insert Size (bp)
    144
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer pQE65, TGAGCGGATAACAATTTCACACAG
  • 3′ sequencing primer pQE276, GGCAACCGAGCGTTCTGAAC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene's NGS results indicate that two copies of SYNZIP1 are present in this plasmid. This plasmid also contains CmR and KanR resistance genes for which functionality not been tested.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQLinkHD-SYNZIP1 was a gift from Amy Keating (Addgene plasmid # 80647 ; http://n2t.net/addgene:80647 ; RRID:Addgene_80647)

  • For your References section:

    SYNZIP protein interaction toolbox: in vitro and in vivo specifications of heterospecific coiled-coil interaction domains. Thompson KE, Bashor CJ, Lim WA, Keating AE. ACS Synth Biol. 2012 Apr 20;1(4):118-29. 10.1021/sb200015u PubMed 22558529
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