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PurposecpstFRET actin sensor for mechanical force measurements in cytoskeleton
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 80643 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameBeta actin, cpstFRET
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Actin-cpstFRET-Actin was a gift from Fred Sachs (Addgene plasmid # 80643 ; http://n2t.net/addgene:80643 ; RRID:Addgene_80643) -
For your References section:
Actin stress in cell reprogramming. Guo J, Wang Y, Sachs F, Meng F. Proc Natl Acad Sci U S A. 2014 Dec 9;111(49):E5252-61. doi: 10.1073/pnas.1411683111. Epub 2014 Nov 24. 10.1073/pnas.1411683111 PubMed 25422450