pet151D-TOPO ALIX Bro1-V (1-702)
(Plasmid #80642)

• Purpose: Residues 1-702 of ALIX; Bacterial Expression Vector; TEV cleavable N-term His-tag; Sundquist Lab Internal ID: WISP10-648
• Depositing Lab: Wesley Sundquist
• Publication: McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105(22):7687-91. doi: 10.1073/pnas.0801567105. Epub 2008 May 29.

Backbone

• Vector backbone: pet151 D-TOPO
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: ALIX Bro1-V
• Species: H. sapiens (human)
• Mutation: Residues 1-702
• Entrez Gene: PDCD6IP (a.k.a. AIP1, ALIX, DRIP4, HP95, MCPH29)
• Tag / Fusion Protein:
  • His Tag (N terminal on backbone)

Cloning Information

• Cloning method: TOPO Cloning
• 5′ sequencing primer: T7 Forward
• 3′ sequencing primer: T7 Reverse

Resource Information

• Supplemental Documents:
  • Genbank File

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry