pet16b ALIX Bro1 (1-359)
(Plasmid #80641)

• Purpose: Residues 1-359 of ALIX; Bacterial Expression Vector; N-term His-tag; Sundquist Lab Internal ID: WISP11-428
• Depositing Lab: Wesley Sundquist
• Publication: McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105(22):7687-91. doi: 10.1073/pnas.0801567105. Epub 2008 May 29.

Backbone

• Vector backbone: pet16b
• Backbone size w/o insert (bp): 5709
• Total vector size (bp): 6786
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: ALIX Bro1, residues 1-359
• Species: H. sapiens (human)
• Insert Size (bp): 1077
• Mutation: Residues 1-359
• Entrez Gene: PDCD6IP (a.k.a. AIP1, ALIX, DRIP4, HP95, MCPH29)
• Promoter: T7
• Tag / Fusion Protein:
  • His Tag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nde1 (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7 Forward
• 3′ sequencing primer: T7 Reverse

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry