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GST-CHMP4A (205-222) L214A (pGEX2T(TEV))
(Plasmid #80639)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 80639 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX 2T (TEV)
  • Backbone manufacturer
    GE
  • Backbone size w/o insert (bp) 4987
  • Total vector size (bp) 5041
  • Modifications to backbone
    TEV site introduced
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CHMP4A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    54
  • Mutation
    Residues 205-222; Changed Leucine 214 to Alanine
  • Entrez Gene
    CHMP4A (a.k.a. C14orf123, CHMP4, CHMP4B, HSPC134, SHAX2, SNF7, SNF7-1, VPS32-1, VPS32A)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nde1 (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer pGEX for
  • 3′ sequencing primer pGEX rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GST-CHMP4A (205-222) L214A (pGEX2T(TEV)) was a gift from Wesley Sundquist (Addgene plasmid # 80639 ; http://n2t.net/addgene:80639 ; RRID:Addgene_80639)

  • For your References section:

    ALIX-CHMP4 interactions in the human ESCRT pathway. McCullough J, Fisher RD, Whitby FG, Sundquist WI, Hill CP. Proc Natl Acad Sci U S A. 2008 Jun 3;105(22):7687-91. doi: 10.1073/pnas.0801567105. Epub 2008 May 29. 10.1073/pnas.0801567105 PubMed 18511562
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