GST-CHMP4B (205-224) (pGEX2T(TEV))
(Plasmid #80634)

• Purpose: Residues 205-224 of CHMP4B, Bacterial Expression Vector; TEV-cleavabel GST tag; Sundquist Lab Internal ID: WISP06-201
• Depositing Lab: Wesley Sundquist
• Publication: McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105(22):7687-91. doi: 10.1073/pnas.0801567105. Epub 2008 May 29.

Backbone

• Vector backbone: pGEX 2T (TEV)
• Backbone manufacturer: GE
• Backbone size w/o insert (bp): 4987
• Total vector size (bp): 5047
• Modifications to backbone: TEV site introduced
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CHMP4B
• Species: H. sapiens (human)
• Insert Size (bp): 60
• Mutation: Residues 205-224
• Entrez Gene: CHMP4B (a.k.a. C20orf178, CHMP4A, CTPP3, CTRCT31, SNF7, SNF7-2, Shax1, VPS32B, Vps32-2, dJ553F4.4)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nde1 (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: pGEX for
• 3′ sequencing primer: pGEX rev

Resource Information

• Supplemental Documents:
  • Genbank File

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry