GST-CHMP4A (pGEX2T(TEV))
(Plasmid #80630)

• Purpose: Bacterial Expression Vector; TEV cleavable GST-tag Internal ID: WISP06-197
• Depositing Lab: Wesley Sundquist
• Publication: McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105(22):7687-91. doi: 10.1073/pnas.0801567105. Epub 2008 May 29.

Backbone

• Vector backbone: pGEX 2T (TEV)
• Backbone manufacturer: GE
• Backbone size w/o insert (bp): 4984
• Total vector size (bp): 5650
• Modifications to backbone: TEV site introduced
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CHMP4A
• Species: H. sapiens (human)
• Insert Size (bp): 666
• Mutation: Residues 1-222
• Entrez Gene: CHMP4A (a.k.a. C14orf123, CHMP4, CHMP4B, HSPC134, SHAX2, SNF7, SNF7-1, VPS32-1, VPS32A)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nde1 (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: pGEX for
• 3′ sequencing primer: pGEX rev

Resource Information

• Supplemental Documents:
  • Genbank File

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry