UAS-Xbp1-GFP
(Plasmid #80627)

• Purpose: expression of Xbp1-GFP (RA isoform) in Drosophila
• Depositing Labs: Pedro Domingos, Herman Steller
• Publication: Ryoo et al EMBO J. 2007 Jan 10;26(1):242-52. Epub 2006 Dec 14.

Backbone

• Vector backbone: pUAST
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Xbp1
• Species: D. melanogaster (fly)
• Mutation: RA isoform
• Entrez Gene: Xbp1 (a.k.a. Dmel_CG9415, 138/3, CG 9415, CG9415, DM18, Dmel\CG9415, Dxbp-1, XBP-1, XBP1, Xbp-1, XbpI, anon-EST:Liang-1.41, clone 1.41, dXbp-1, dmXBP1, l(2)k13803, sXbp1, xbp-1, xbp1)
• Promoter: UAS
• Tag / Fusion Protein:
  • EGFP (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: pCasper-hs
• 3′ sequencing primer: EGFP-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

uas-xbp1-EGFP was made by subcloning EGFP after the EcoRV site of xbp1, which generated a chimeric construct with EGFP fused after the 252nd amino-acid residue of the xbp1-RA isoform.