pEB1deltaC-mCherry
(Plasmid #80603)

• Purpose: Expresses C-terminally truncated, mCherry-tagged EB1 as marker for growing microtubule plus end
• Depositing Lab: Torsten Wittmann
• Publication: Ettinger et al Curr Biol. 2016 Jun 20;26(12):1549-55. doi: 10.1016/j.cub.2016.04.020. Epub 2016 May 26.

Backbone

• Vector backbone: pmCherry-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: EB1
• Alt name: MAPRE1
• Species: H. sapiens (human)
• Insert Size (bp): 747
• Mutation: Truncated C-terminus; EB1 coding sequence ends at threonine 249
• GenBank ID: NM_012325
• Entrez Gene: MAPRE1 (a.k.a. EB1)
• Promoter: CMV
• Tag / Fusion Protein:
  • mCherry (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nhe1 (not destroyed)
• 3′ cloning site: Not1 (not destroyed)
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: mCherry reverse

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry