pWHI2-HYG
(Plasmid
#80589)
-
PurposeCEN plasmid expressing full-length WHI2 from S.cerevisiae S288C background under its native promoter
-
Depositing Lab
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 80589 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepRS316/pAG36
- Backbone size w/o insert (bp) 6568
- Total vector size (bp) 8688
-
Vector typeYeast Expression
-
Selectable markersHygromycin, URA3
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert nameWHI2
-
SpeciesS. cerevisiae (budding yeast)
-
Insert Size (bp)2100
-
GenBank ID
-
Entrez GeneWHI2 (a.k.a. YOR043W)
- Promoter WHI2 native promoter
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site PacI (not destroyed)
- 5′ sequencing primer ACGGGCCATGTTCAATGGC (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pWHI2-HYG was a gift from John McCusker (Addgene plasmid # 80589 ; http://n2t.net/addgene:80589 ; RRID:Addgene_80589) -
For your References section:
Mitochondrial Genome Variation Affects Multiple Respiration and Non-respiration Phenotypes in Saccharomyces cerevisiae. Vijayraghavan S, Kozmin SG, Strope PK, Skelly DA, Lin Z, Kennell J, Magwene PM, Dietrich FS, McCusker JH. Genetics. 2018 Nov 29. pii: genetics.118.301546. doi: 10.1534/genetics.118.301546. 10.1534/genetics.118.301546 PubMed 30498022
Map uploaded by the depositor.