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Addgene

pWHI2-NAT
(Plasmid #80588)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 80588 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS316/pAG36
  • Backbone size w/o insert (bp) 6000
  • Total vector size (bp) 8228
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3 ; NAT (select with Nourseothricin/clonNAT)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    WHI2
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    2120
  • GenBank ID
    NM_001183462.1
  • Entrez Gene
    WHI2 (a.k.a. YOR043W)
  • Promoter Native WHI2 promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site PacI (not destroyed)
  • 5′ sequencing primer ACGGGCCATGTTCAATGGC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWHI2-NAT was a gift from John McCusker (Addgene plasmid # 80588 ; http://n2t.net/addgene:80588 ; RRID:Addgene_80588)

  • For your References section:

    Mitochondrial Genome Variation Affects Multiple Respiration and Non-respiration Phenotypes in Saccharomyces cerevisiae. Vijayraghavan S, Kozmin SG, Strope PK, Skelly DA, Lin Z, Kennell J, Magwene PM, Dietrich FS, McCusker JH. Genetics. 2018 Nov 29. pii: genetics.118.301546. doi: 10.1534/genetics.118.301546. 10.1534/genetics.118.301546 PubMed 30498022
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