pME-FusionRed-NS
(Plasmid
#80500)
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PurposeMultisite gateway entry clone for adding N-terminal fusions of FusionRed
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 80500 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDONR221
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Vector typeMultisite gateway middle entry clone
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFusionRed (no STOP)
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SpeciesSynthetic
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pME-FusionRed-NS was a gift from Rob Parton (Addgene plasmid # 80500 ; http://n2t.net/addgene:80500 ; RRID:Addgene_80500) -
For your References section:
A plasmid library of full-length zebrafish rab proteins for in vivo cell biology. Hall TE, Martel N, Lo HP, Xiong Z, Parton RG. Cell Logist. 2017 Mar 1;7(1):e1301151. doi: 10.1080/21592799.2017.1301151. eCollection 2017. 10.1080/21592799.2017.1301151 PubMed 28396820
Map uploaded by the depositor.
