pAAVS1-P-CAG-mCh
(Plasmid #80492)

• Purpose: donor vector for AAVS1 targeting (puromycin selection) and constitutive mCherry expression
• Depositing Lab: Knut Woltjen
• Publication: Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15)30181-X. doi: 10.1016/j.ymeth.2015.12.012.

Backbone

• Vector backbone: pBluescript
• Backbone size w/o insert (bp): 3000
• Modifications to backbone: Homology arms for AAVS1; promoter-less SA-T2A-puro selection cassette
• Vector type: Mammalian Expression ; donor vector (human)
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mCherry
• Species: Synthetic
• Insert Size (bp): 711
• Promoter: CAG

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: AGCCTCTGCTAACCATGTTC
• 3′ sequencing primer: ATTAGCCAGAAGTCAGATGC

Resource Information

• Supplemental Documents:
  • Genbank
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Deleted T2 protospacer sequence in HA-R