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Purpose(Empty Backbone) All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (constitutive rtTA and puromycin resistance)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
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| Plasmid | 80477 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBluescript
- Backbone size (bp) 3000
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Vector typeMammalian Expression ; piggyBac transposon
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Requires co-transfection with a piggyBac transposase plasmid for stable genomic integration. If you desire to use transposase with the Materials, you may contact Transposagen or other licensed vendor.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PB-TA-ERP2 was a gift from Knut Woltjen (Addgene plasmid # 80477 ; http://n2t.net/addgene:80477 ; RRID:Addgene_80477) -
For your References section:
Inducible Transgene Expression in Human iPS Cells Using Versatile All-in-One piggyBac Transposons. Kim SI, Oceguera-Yanez F, Sakurai C, Nakagawa M, Yamanaka S, Woltjen K. Methods Mol Biol. 2016;1357:111-31. doi: 10.1007/7651_2015_251. 10.1007/7651_2015_251 PubMed 26025620
Map uploaded by the depositor.
