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Addgene

PB-TAG-ERN
(Plasmid #80476)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 80476 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript
  • Backbone size (bp) 3000
  • Vector type
    Mammalian Expression ; piggyBac transposon
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Requires co-transfection with a piggyBac transposase plasmid for stable genomic integration. If you desire to use transposase with the Materials, you may contact Transposagen or other licensed vendor.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB-TAG-ERN was a gift from Knut Woltjen (Addgene plasmid # 80476 ; http://n2t.net/addgene:80476 ; RRID:Addgene_80476)

  • For your References section:

    Inducible Transgene Expression in Human iPS Cells Using Versatile All-in-One piggyBac Transposons. Kim SI, Oceguera-Yanez F, Sakurai C, Nakagawa M, Yamanaka S, Woltjen K. Methods Mol Biol. 2016;1357:111-31. doi: 10.1007/7651_2015_251. 10.1007/7651_2015_251 PubMed 26025620
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