pX330-Flag-HeFSpCas9-H840A
(Plasmid #80456)

• Purpose: high fidelity enhanced SpCas9 mammalian expression vector
• Depositing Lab: Ervin Welker
• Publication: crispr/cas9 (unpublished)

Backbone

• Vector backbone: pX330
• Backbone size w/o insert (bp): 3922
• Total vector size (bp): 8074
• Vector type: Mammalian Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HeFSpCas9_H840A
• Alt name: High Fidelity Enhanced Streptococcus pyogenes Cas9 nuclease
• Species: Streptococcus pyogenes
• Insert Size (bp): 4152
• Promoter: CBh
• Tags / Fusion Proteins:
  • 3xFLAG (N terminal on insert)
  • NLS (N terminal on insert)
  • NLS (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: TAGAAGGCACAGTCGAGG

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry