pCIG-FGFRdn
(Plasmid #80431)

• Purpose: Expression of a dominant-negative FGFR1 with a nuclear EGFP reporter in chick embryos
• Depositing Lab: Aixa Morales
• Publication: Martinez-Morales et al J Cell Biol. 2011 Aug 8;194(3):489-503. doi: 10.1083/jcb.201011077. Epub 2011 Aug 1.

Backbone

• Vector backbone: pCIG
• Backbone size w/o insert (bp): 6000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Fibroblast growth factor receptor 1
• Alt name: FGFR1
• Species: G. gallus (chicken)
• Mutation: contains aa 1–425
• Entrez Gene: FGFR1
• Promoter: beta-actin promoter
• Tag / Fusion Protein:
  • EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: pCAG-F
• 3′ sequencing primer: pCDH-rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pW1Act-VP16.hRARα insert was originally provided by Michael Underhill. We subsequently cloned this insert into PCIG.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry