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Addgene

pSMART-sgRNA (Sp)
(Plasmid #80427)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 80427 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PSMARTHCKan
  • Backbone manufacturer
    Lucigen
  • Modifications to backbone
    Insertion of U6 promoter, trcerRNA and two BbsI sites
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert complementary annealed oligonucleotides encoding desired crRNA into BbsI sites. Include following overhangs:

5' ODN: CACCG(N19)
3' ODN: AAAC(N19)C

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSMART-sgRNA (Sp) was a gift from Sara Howden & Melissa Little (Addgene plasmid # 80427 ; http://n2t.net/addgene:80427 ; RRID:Addgene_80427)

  • For your References section:

    A Cas9 Variant for Efficient Generation of Indel-Free Knockin or Gene-Corrected Human Pluripotent Stem Cells. Howden SE, McColl B, Glaser A, Vadolas J, Petrou S, Little MH, Elefanty AG, Stanley EG. Stem Cell Reports. 2016 Sep 13;7(3):508-17. doi: 10.1016/j.stemcr.2016.07.001. Epub 2016 Aug 4. 10.1016/j.stemcr.2016.07.001 PubMed 27499201
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