pDNR-SpCas9-Cdt1
(Plasmid
#80426)
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PurposeUsed for in vitro transcription of SpCas9-Cdt1 mRNA. Cas9 is fused to Cdt1 peptide and is degraded during S/G2 phases of the cell-cycle.
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 80426 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDNR-Dual
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Backbone manufacturerClontech
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsMay get better pDNA yield if grown at 30 degrees
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameSpCas9-Cdt1
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SpeciesS.pyogenes
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Insert Size (bp)4479
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Mutationcodon optimized for human
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Tag
/ Fusion Protein
- Cdt1 (C terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDNR-SpCas9-Cdt1 was a gift from Sara Howden & Melissa Little (Addgene plasmid # 80426 ; http://n2t.net/addgene:80426 ; RRID:Addgene_80426) -
For your References section:
A Cas9 Variant for Efficient Generation of Indel-Free Knockin or Gene-Corrected Human Pluripotent Stem Cells. Howden SE, McColl B, Glaser A, Vadolas J, Petrou S, Little MH, Elefanty AG, Stanley EG. Stem Cell Reports. 2016 Sep 13;7(3):508-17. doi: 10.1016/j.stemcr.2016.07.001. Epub 2016 Aug 4. 10.1016/j.stemcr.2016.07.001 PubMed 27499201
