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Addgene

pDNR-SpCas9
(Plasmid #80425)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 80425 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDNR-Dual
  • Backbone manufacturer
    Clontech
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    May get better pDNA yield if grown at 30 degrees
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    SpCas9
  • Species
    S.pyogenes
  • Insert Size (bp)
    4104
  • Mutation
    codon optimized for human
  • Promoter T7
  • Tag / Fusion Protein
    • none

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (destroyed during cloning)
  • 3′ cloning site NsiI (destroyed during cloning)
  • 5′ sequencing primer taatacgactcactatag
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDNR-SpCas9 was a gift from Sara Howden & Melissa Little (Addgene plasmid # 80425 ; http://n2t.net/addgene:80425 ; RRID:Addgene_80425)

  • For your References section:

    A Cas9 Variant for Efficient Generation of Indel-Free Knockin or Gene-Corrected Human Pluripotent Stem Cells. Howden SE, McColl B, Glaser A, Vadolas J, Petrou S, Little MH, Elefanty AG, Stanley EG. Stem Cell Reports. 2016 Sep 13;7(3):508-17. doi: 10.1016/j.stemcr.2016.07.001. Epub 2016 Aug 4. 10.1016/j.stemcr.2016.07.001 PubMed 27499201
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