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AAV-flex-plap
(Plasmid #80422)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 80422 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV
  • Total vector size (bp) 6770
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    plap
  • Alt name
    alkaline phosphatase, placental
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1413
  • Entrez Gene
    ALPP (a.k.a. ALP, ALPI, IAP, PALP, PLAP, PLAP-1)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer CMV_fwd_primer
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-flex-plap was a gift from Nirao Shah (Addgene plasmid # 80422 ; http://n2t.net/addgene:80422 ; RRID:Addgene_80422)

  • For your References section:

    Retinofugal Projections from Melanopsin-Expressing Retinal Ganglion Cells Revealed by Intraocular Injections of Cre-Dependent Virus. Delwig A, Larsen DD, Yasumura D, Yang CF, Shah NM, Copenhagen DR. PLoS One. 2016 Feb 19;11(2):e0149501. doi: 10.1371/journal.pone.0149501. eCollection 2016. 10.1371/journal.pone.0149501 PubMed 26895233
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