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Addgene

pBItetDT12nGFP
(Plasmid #80420)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 80420 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBiTet
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFP and human DMPK exons 11-15 with 12 CTG repeats
  • Species
    H. sapiens (human)
  • Entrez Gene
    DMPK (a.k.a. DM, DM1, DM1PK, DMK, MDPK, MT-PK)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that there are some discrepancies between Addgene's quality control sequences and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBItetDT12nGFP was a gift from Thomas Cooper (Addgene plasmid # 80420 ; http://n2t.net/addgene:80420 ; RRID:Addgene_80420)

  • For your References section:

    RNase H-mediated degradation of toxic RNA in myotonic dystrophy type 1. Lee JE, Bennett CF, Cooper TA. Proc Natl Acad Sci U S A. 2012 Mar 13;109(11):4221-6. doi: 10.1073/pnas.1117019109. Epub 2012 Feb 27. 10.1073/pnas.1117019109 PubMed 22371589
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