DT12A
(Plasmid #80416)

• Purpose: Expresses 12 CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats
• Depositing Lab: Thomas Cooper
• Publication: Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub 2004 Jul 15.

Backbone

• Vector backbone: sp72
• Backbone size w/o insert (bp): 2400
• Total vector size (bp): 5487
• Modifications to backbone: the interrupted repeats contain 20 CTGs with TCGAC interruptions where SalI and XhoI sites were ligated to concatermize the sets of 20 repeats
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human DMPK exons 11-15 with 12 CTG repeats
• Species: H. sapiens (human)
• Insert Size (bp): 3162
• Entrez Gene: DMPK (a.k.a. DM, DM1, DM1PK, DMK, MDPK, MT-PK)

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that there are some discrepancies between Addgene's quality control sequences and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.