R300TA
(Plasmid
#80411)
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Purposehuman cTNT minigene with genomic segment containing alternative exon 5 with CELF1 binding site mutated (CTGs->CAGs)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 80411 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneBluescript KS+
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTNNT2
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SpeciesH. sapiens (human)
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Insert Size (bp)4300
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MutationCTGs downstream of alternative exon mutated to CAGs
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Entrez GeneTNNT2 (a.k.a. CMD1D, CMH2, CMPD2, LVNC6, RCM3, TnTC, cTnT)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
R300TA was a gift from Thomas Cooper (Addgene plasmid # 80411 ; http://n2t.net/addgene:80411 ; RRID:Addgene_80411) -
For your References section:
Disruption of splicing regulated by a CUG-binding protein in myotonic dystrophy. Philips AV, Timchenko LT, Cooper TA. Science. 1998 May 1;280(5364):737-41. PubMed 9563950