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Addgene

Stargazin-GFP-LOVpep
(Plasmid #80406)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 80406 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    unknown
  • Backbone size w/o insert (bp) 5183
  • Total vector size (bp) 7235
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Stargazin-GFP-LOVpep
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2412
  • Entrez Gene
    CACNG2 (a.k.a. MRD10)
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GATGGGGCTGTTTGATCGAGGTG
  • 3′ sequencing primer AAACGGGCCCTCTAGgtgacataactaattacatgactcgaG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Stargazin-GFP-LOVpep was a gift from Michael Glotzer (Addgene plasmid # 80406 ; http://n2t.net/addgene:80406 ; RRID:Addgene_80406)

  • For your References section:

    Local RhoA activation induces cytokinetic furrows independent of spindle position and cell cycle stage. Wagner E, Glotzer M. J Cell Biol. 2016 Jun 20;213(6):641-9. doi: 10.1083/jcb.201603025. Epub 2016 Jun 13. 10.1083/jcb.201603025 PubMed 27298323
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